Elisa Kit Test Fault tih famkim dan tur zirtir ang che?

ELISA operation process hi a tam hle a, novice operation hian tihsual a nei ngei ang. Tin, heng tihsual tam tak hi a chipchiar zawnga siam a ni a, a tha tawk lo a, a dik lohna tihziaawmna tur kawng tam tak a awm a, chu chu thusawi tlem zawk tur experiment tih te, enzyme label chhunga saliva tlak loh nan te a ni. Dik tak chuan, buaina laih chhuah zir a ngai a, a chhan zawng chhuak tur a ni. Vawiin hian Elisa Kit Test Fault tih famkim dan tur zirtir rawh?

1, reagent hman theih dan, reagent stability, concave leh convex dikna te endik a pawimawh. Reagent hman hmain yin leh yang control leh sample repeated comparison test te chu hman hmain reagent hian a mamawh ang a tlin tih hriat a ngai a ni.

2. Hman hmain ngun takin thupek chhiar la, thupek angin standardized operation khauh takin kalpui rawh. Reagents batch hrang hrang te chu inhmeh loh tur a ni. A hmun siam\\hat tlak a nih chuan, a dinhmun siam \\hat a nih leh nih loh finfiah turin test chu ti leh la, chu chu washing board grasp zat ang chi a ni.

3. Sample dik tak leh rang takin add rawh. Sample dik lo dah belh a nih chuan enzyme products awm zat chu finfiah theih a ni lo a, direct takin color result a nghawng a ni. Chu bakah, depth of color leh value tehna chu color agent dah belh leh stop liquid zat nen a inzawm a, chuvangin add sample te chu fimkhur a ngai hle. Sample chu hun bituk chhunga dah a nih chuan, sample chu zawi zawiin a dah chuan error chu a awm ang a, reagent chu hun rei tak a lang ang a, a bik takin room temperature a san lutuk chuan shelf life chu a tawi ang a, a hlawhtling lo thei bawk ang.

4, inspection technician chuan laboratory operation-a hman tur quality leh practical experience bulpui a nei tur a ni. Test instrument leh instrument thiam taka hman theihna, harsatnate khaikhawm leh thlirlet theihna nei, leh test-a beisei loh dinhmunte a hun taka leh a hun taka chinfel theihna nei.

5, calibrated micro pipette hman hian natural error a ti bo. Pipette dikna hi quantitative detection atan a pawimawh hle.

6, Strictly Master the Color Time, Color Time a tawi lutuk a, stop liquid reaction stop add la, substrate binder a tlem lutuk, false negative anga lang awlsam. Color development atana hun mamawh chu a pelh chuan a result chu false positive a ni tur a ni a, chu chu reagent ngei nen pawh a inzawm thei a ni. Chromogenic hnu lawkah chromogenic agent dah nghal la, positive ni lovin, hei hi background chromogenic atanga lo chhuak a ni thei.

7, Wash vek, wash plate a kim lo, ELISA kit enzyme conjugate background color chu false positive angin a lang ang. Chu bakah, cleaning solution chu a thar tur a ni a, tunah chuan, stale cleaning solution chu background heightening phenomenon angin a lang ang a, false positive angin a lang thei bawk.

8, reaction time chu strict takin control la, reaction time a rei lutuk, enzyme inactivation; Reaction time a tawi lutuk a, enzyme conjugate chu microbial antigen leh serum-a antibody nen a inzawm kim thei lo va, product structure chu a lo chhuak a, a nghet lo a, silfai a awlsam a, false negative a siam thei bawk.